Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/22

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E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/10/22 Friday (Naoto)

member naoto and watachin

Experiment:Colony PCR

material

  • colony of E.coli
    • bcsA:1~22
    • bcsD:25~48

other materials were same as protocol3

procedure

see protocol3

Experiment:Electrophoresis

material

  • PCR production (Colony PCR)

you can see other materials at protocol4

procesure

refer to protocol4

result

From the length of bands

bcsA→No.2,9,11,

seem to be correct inserts

Experiment:Colony PCR=

material

  • colony of E.coli
    • bcsB:1~24
    • bcsC:25~48

other materials were same as protocol3

procedure

see protocol3

Experiment:Electrophoresis

material

  • PCR production (Colony PCR)

you can see other materials at protocol4

procesure

refer to protocol4

result

we couldn't confirm insert



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