From 2010.igem.org
E.coli Fiber Project Notebook
2010/10/21 Thursday (Naoto)
- member
- naoto and watachin
Experiment:PCR
- material
- templete DNA (from A.xylinum and pSB1C3)
- you can see other materials at protocol3
- procedure
- see protocol3
Experiment:Electrophoresis
- material
- PCR production(bcsA and bcsB)
- you can see materials at protocol4
- procedure
- refer to protocol4
Experiment:DNA purification with silica gel
- material
- PCR production(bcsD and pSB1C3)
- you can see materials at protocol5-2
- procedure
- refer to protocol5-2
Experiment:Electrophoresis
- material
- PCR production (bcsA,bcsB,bcsC and bcsD)
- you can see other materials at protocol4
- procedure
- see protocol4
Experiment:Digestion
- material
- you can see other materials at protocol6
- procedure
- see protocol6
Experiment:DNA purification with silica gel
- material
- PCR production(bcsC and bcsD)
- you can see materials at protocol5-2
- procedure
- refer to protocol5-2
Experiment:Ligation
- material
- you can see materials at protocol7
- procedure
- refer to protocol7
Experiment:Transformation
- material
- Competent cell(Ecos JM109)
- bcsD in pSB1C3
- you can see materials at protocol8
- procedure
- see protocol8
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