Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/16

From 2010.igem.org


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/09/16 Thuesday (Bambi75)

Experiment:PCR

Member
naoto and bambi75
Materials,Procedure
Same protocol4
  • 95℃3min
  • 96℃1min
  • 51℃1min
  • 72℃3min
  • 10℃∞
（96℃～72℃/30cycle）

Experiment:Agarose gel Electrophoresis

Member
Same above
Materials,Procedure
Same protocol4
  • PCR production (pSB1C3)

Preparation:Make Agarose Gel Electrophoresis

Member
Same above
Materials and Procedure
Same protocol4

Preparation:Medium making

Member
Same above
Materials and Procedure
Same protocol1

Experiment:Ligation

Member
Same above
Materials and Procedure
follow protocol8
  • bcsA,B,C and D
  • pSB1C3

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