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Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/22
From 2010.igem.org
E.coli Fiber Project Notebook
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2010/10/22 Friday (Naoto)
- member
- naoto and watachin
Experiment:Colony PCR
- material
- colony of E.coli
- bcsA:1~22
- bcsD:25~48
- colony of E.coli
- other materials were same as protocol3
- procedure
- see protocol3
Experiment:Electrophoresis
- material
- PCR production (Colony PCR)
- you can see other materials at protocol4
- procesure
- refer to protocol4
- result
- From the length of bands
- bcsA→No.2,9,11,
- seem to be correct inserts
Preparation:making preculture for Miniprep
- material
- colony of E.coli
- bcsA→No.2,9,11
- LB+Cam broth
- colony of E.coli
- procedure
- see protocol2
Experiment:Colony PCR
- material
- colony of E.coli
- bcsB:1~24
- bcsC:25~48
- colony of E.coli
- other materials were same as protocol3
- procedure
- see protocol3
Experiment:Electrophoresis
- material
- PCR production (Colony PCR)
- you can see other materials at protocol4
- procesure
- refer to protocol4
- result
- we couldn't confirm inserts
