Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/18

E.coli Fiber Project Notebook

August 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

September 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

October 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

2010/09/18 Saturday (Naoto)

Experiment:Electrophoresis

Member

naoto and easily

Material

• PCR production(pSB1C3)

If you want to know other materials,refer to protocol4

Procedure

follow to protocol3

Result

Bands didn't appear

Experiment:Colony PCR

Member

naoto and easily

Material

• Parts of bcsA,B,C,D(A.xylinum) bcsA,B,C(E.coli)in colonies of E,coli

If you want to know other materials,refer to protocol3

Procedure

follow to protocol3

Experiment:PCR

Member

naoto and easily

Material

• bcsA,B,C,D(A.xylinum),pSB1C3

If you want to know other materials,refer to protocol3

Procedure

follow to protocol3

Experiment:Electrophoresis of bcsA,B,C,D(A.xylinum),pSB1C3

Member

naoto and easily

Material

• PCR production(bcsA,B,C,D(A.xylinum),bcsA,B,C(E.coli),pSB1C3)

If you want to know other materials,refer to protocol4

Procedure

follow to protocol3

Result

Only band of bcsA(E.coli) could be seen

Experiment:DNA Extraction from agarose gel

Member

naoto and easily

Material

• bcsA(E.coli) in agarose gel

If you want to know other materials,refer to protocol5

Procedure

follow to protocol5

Experiment:Electrophoresis of Colony PCR

Member

naoto and easily

Material

• PCR production (parts of bcsA,B,C,D(A.xylinum) and bcsA(E.coli)in colonies of E,coli)

If you want to know other materials,refer to protocol4

Procedure

refer to protocol4

Result

Bands of bcsB,C(A.xylinum) appreared

• 1~8 bcsA(A.xylinum)
• 9~12 bcsB(A.xylinum)
• 13 bcsC(A.xylinum)
• 14~15 bcsD(A.xylinum)
• 16~20 bcsA(E.coli)