Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/13

From 2010.igem.org


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/08/13 Friday (bambi75)

Experiment:Restrict enzyme digestion

Member
NEX , bambi75 and watachin
Materials
  • bcsA,B,C,D(each):50μl
  • 10×M buffer:15μl
  • BSA:15μl
  • XbaI:3μl
  • SpeI:3μl
Procedure
  1. mix materials : M buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
  2. incubation(37℃).