Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/16

From 2010.igem.org


E.coli Fiber Project Notebook

August 2010
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2010/10/16 Saturday (Naoto)

member
naoto and watachin

Experiment:Electrophoresis

material
  • PCR production (bcsA,bcsB,bcsC and bcsD)
you can see other materials at protocol4
procedure
see protocol4

Experiment:Digestion

material
  • bcsD
  • XbaI
  • SpeI
you can see other materials at protocol6
procedure
see protocol6

Experiment:DNA purification with silica gel

material
  • PCR production(bcsD)
you can see materials at protocol5-2
procedure
refer to protocol5-2

Experiment:Ligation

material
  • bcsD
  • pSB1C3
you can see materials at protocol7
procedure
refer to protocol7

Experiment:Transformation

material
  • Competent cell(NovaBlue)
  • bcsD in pSB1C3
you can see materials at protocol8
procedure
see protocol8



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