Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/22
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Revision as of 17:21, 25 October 2010
E.coli Fiber Project Notebook
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2010/10/22 Friday (Naoto)
member naoto and watachin
Experiment:Colony PCR
material
- colony of E.coli
- bcsA:1~22
- bcsD:25~48
other materials were same as protocol3
procedure
see protocol3
Experiment:Electrophoresis
material
- PCR production (Colony PCR)
you can see other materials at protocol4
procesure
refer to protocol4
result
From the length of bands
bcsA→No.2,9,11,
seem to be correct inserts
Experiment:Colony PCR
material
- colony of E.coli
- bcsB:1~24
- bcsC:25~48
other materials were same as protocol3
procedure
see protocol3
Experiment:Electrophoresis
material
- PCR production (Colony PCR)
you can see other materials at protocol4
procesure
refer to protocol4
result
we couldn't confirm insert