Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/22

From 2010.igem.org

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==2010/10/22 Friday (Naoto)==
 +
'''member'''
 +
naoto and watachin
 +
 +
===Experiment:Colony PCR===
 +
'''material'''
 +
 +
*colony of E.coli
 +
**bcsA:1~22
 +
**bcsD:25~48
 +
other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
 +
 +
'''procedure'''
 +
 +
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
 +
 +
===Experiment:Electrophoresis===
 +
'''material'''
 +
 +
*PCR production (Colony PCR)
 +
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
 +
 +
'''procesure'''
 +
 +
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
 +
 +
'''result'''
 +
 +
From the length of bands
 +
 +
bcsA→No.2,9,11,
 +
 +
seem to be correct inserts
 +
 +
==Experiment:Colony PCR===
 +
'''material'''
 +
 +
*colony of E.coli
 +
**bcsB:1~24
 +
**bcsC:25~48
 +
other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
 +
 +
'''procedure'''
 +
 +
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
 +
 +
===Experiment:Electrophoresis===
 +
'''material'''
 +
 +
*PCR production (Colony PCR)
 +
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
 +
 +
'''procesure'''
 +
 +
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
 +
 +
'''result'''
 +
 +
we couldn't confirm insert
 +
 +
 +
 +
<br/>

Revision as of 17:21, 25 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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22 23 24 25 26 27 28
29 30 31
September 2010
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October 2010
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31

Contents

2010/10/22 Friday (Naoto)

member naoto and watachin

Experiment:Colony PCR

material

  • colony of E.coli
    • bcsA:1~22
    • bcsD:25~48

other materials were same as protocol3

procedure

see protocol3

Experiment:Electrophoresis

material

  • PCR production (Colony PCR)

you can see other materials at protocol4

procesure

refer to protocol4

result

From the length of bands

bcsA→No.2,9,11,

seem to be correct inserts

Experiment:Colony PCR=

material

  • colony of E.coli
    • bcsB:1~24
    • bcsC:25~48

other materials were same as protocol3

procedure

see protocol3

Experiment:Electrophoresis

material

  • PCR production (Colony PCR)

you can see other materials at protocol4

procesure

refer to protocol4

result

we couldn't confirm insert



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