User contributions
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- 21:25, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/14 September 2010 (New page: == Module I, group 2c == Repeat the PCR of yesterday.) (top)
- 21:24, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/13 September 2010 (New page: == Module I, group 2c == Since we have got no positive results for mutiple-fragment ligation, we really want to know the efficiency of it. Therefore, we use the ligation product as templa...) (top)
- 21:12, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/4 September 2010 (top)
- 21:06, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/3 September 2010 (New page: == Module I, group 2c == Ligation product is transformed in to DH5aα 24h after ligation.) (top)
- 21:05, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/2 September 2010 (→YX's part) (top)
- 21:01, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/1 September 2010 (top)
- 20:57, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/31 August 2010 (→Module I, group 2c) (top)
- 20:50, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/31 August 2010 (→Module I, group 2c)
- 20:50, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/31 August 2010
- 20:47, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/27 August 2010 (New page: == Module I, group 2c == As too many negative clones grown on the control plate, which means that vector has not been toally digested. To solve the problem, we decide to use gel purificati...) (top)
- 20:37, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/26 August 2010 (→Module I, Group 2c) (top)
- 20:34, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/24 August 2010 (→Module I, Group 2b) (top)
- 20:28, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/25 August 2010 (→Module I, group 2(b)) (top)
- 20:27, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/26 August 2010
- 20:25, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/24 August 2010 (→Module I, Group 2b)
- 20:20, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/23 August 2010 (→Module I, group 2c) (top)
- 20:19, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/23 August 2010 (→Module I, group 2c)
- 19:48, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/24 August 2010 (New page: == Module I, Group 2b == After purification, ligation reaction took place in a mixed system as following. eGFP 1ul mCherry 2ul Kan 1ul T4...)
- 19:43, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/16 August 2010 (→Module I, group 2c) (top)
- 19:43, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/23 August 2010 (→Module I, group 2c)
- 19:42, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/23 August 2010 (New page: == Module I, group 2c == Run the same PCR program as yesterday and purify the product with gel purification kit. ---- Digest eGFP with XbaI and EcoRI, Ap is uesd to phosphorylate the 5' e...)
- 19:36, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/16 August 2010 (→Module I, group 2c)
- 19:34, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/21 August 2010 (top)
- 19:30, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/21 August 2010 (New page: == Module I, group 2c == == Module I, group 2c == Extract plasmid from the clone we Picked yesterday. Digest the plasmid with EcoRI and run gel to identify whether the clone is a positiv...)
- 19:12, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/20 August 2010 (top)
- 19:10, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/20 August 2010 (New page: == Module I, group 2c == Extract plasmid from the clone we Picked yesterday. Digest the plasmid with EcoRI and run gel to identify whether the clone is a positive one. digestion system ...)
- 19:06, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/19 August 2010 (top)
- 19:05, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/18 August 2010 (top)
- 19:04, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/17 August 2010
- 18:50, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/16 August 2010
- 18:37, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/7 August 2010 (→Module I, group 2c) (top)
- 18:35, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/7 August 2010 (→Module I, group 2(b))
- 17:10, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/2 August 2010 (top)
- 16:58, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/31 July 2010 (→Module I, Group 2c) (top)
- 16:57, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/1 August 2010 (top)
- 16:43, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/31 July 2010 (New page: == Module I, Group 2c == To solve the problem of severe dimerization of primers, we tried to adjust PCR system and use different concentration of upstream and downstream primers. As the c...)
- 07:45, 25 October 2010 (diff | hist) N File:10.7.31 PCR recover gel.tif (top)
- 07:36, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/30 July 2010 (→Module I, Group 2c) (top)
- 07:32, 25 October 2010 (diff | hist) N Team:Tsinghua/Notebook/30 July 2010 (New page: == Module I, Group 2c == To get target fragments with higher concentration, PCR and gel purification is conducted. PCR system and program is the same as what I used yesterday. ---- Resul...)
- 07:27, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/26 July 2010 (→2c) (top)
- 07:25, 25 October 2010 (diff | hist) N Team:Tsinghua/Notebook/29 July 2010 (New page: == Module I, Group 2c == Transformation is conducted to amplify the plasmid containing gene mcherry, which is used as template for PCR. ---- PCR system (Prime Star): H2O ...) (top)
- 07:18, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/27 July 2010 (→Group 2c) (top)
- 07:17, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/27 July 2010 (→Module I, Danyang's part:)
- 07:16, 25 October 2010 (diff | hist) Team:Tsinghua/Notebook/26 July 2010 (→Module II, Danyang's part:)
- 07:14, 14 September 2010 (diff | hist) Team:Tsinghua (→Unser Projekt)
- 09:45, 11 September 2010 (diff | hist) Team:Tsinghua (→Our Project)
- 12:59, 3 September 2010 (diff | hist) Team:Tsinghua/Notebook/27 July 2010 (→Module I, DT and Fan's part:)
- 12:52, 3 September 2010 (diff | hist) N Team:Tsinghua/Notebook/26 July 2010 (New page: == Module II, Danyang's part: == To test the effectivity of newly-synthetized, PCR assay to amplify mCherry, Kan and eGFP, Chlr is conducted. PCR system (FastPFU): H2O ...)
- 08:10, 16 July 2010 (diff | hist) Team:Tsinghua/team
- 11:52, 30 May 2010 (diff | hist) Team:Tsinghua
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