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From 2010.igem.org

Module I, Group 2c

To get target fragments with higher concentration, PCR and gel purification is conducted. PCR system and program is the same as what I used yesterday.

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Result: to my disappoint, after gel purification,target fragments disappeared! The concentration of fragments is less than 10ng/ul, which is far from enough for following digestion and ligation. Besides, dimerization of primers is severe.