Team:Tsinghua/Notebook/31 August 2010


YX's part

Picked three clones of each resistance gene and shook in 3ml LB media.

Extracted the plasmid and did PCR to verify the sequence.

The positive clones were sent for sequencing.

Module I, group 2c

Digest plasmid PSB1C13 with XbaI and SpeI, AP is used to dephosphorylate the 5' end of digestion product.

digestion system----

PSB1C13        15ul
10X M buffer    2ul
XbaI           1.5ul
SpeI           1.5ul

Incubate at 37°C overnight.