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From 2010.igem.org

YX's part

PCR amplified the second round of the resistance genes.

Ligated to T vectors as before.

Module I, group 2c

12h after digestion, run a gel and cut the band conaining digested vector and purify it with kit.

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Measure the concentration of the purified product.

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Dephosphorylate the digested vector with AP.

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system

AP            1ul
Buffer        2ul
plasmid       17ul

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Incubate at 37°C for 2h and then purify the product with TIANGEN kit.