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From 2010.igem.org

YX's part

PCR amplified the replication origin from pTKS/CS.

Ligated to T vector as before.

Transformed trans1-T1 and spread on Kanamycin LB plates

Module I, group 2c

Pick several clones and culture them in 3ml LB with Amp respectively.

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Extract plasmid from the clone we Picked yesterday. Digest the plasmid with EcoRI and run gel to identify whether the clone is a positive one.

digestion system

plasmid            6ul
EcoRI              1ul
FD Buffer          2ul
H2O                11ul

Result: None of the clone picked is positive.