Team:Tsinghua/Notebook/4 September 2010


YX's part

PCR amplified the replication origin from pTKS/CS.

Ligated to T vector as before.

Transformed trans1-T1 and spread on Kanamycin LB plates

Module I, group 2c

Pick several clones and culture them in 3ml LB with Amp respectively.

Extract plasmid from the clone we Picked yesterday. Digest the plasmid with EcoRI and run gel to identify whether the clone is a positive one.

digestion system

plasmid            6ul
EcoRI              1ul
FD Buffer          2ul
H2O                11ul

Result: None of the clone picked is positive.