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From 2010.igem.org

Module I, group 2c

Extract plasmid from the clone we Picked yesterday. Digest the plasmid with EcoRI and run gel to identify whether the clone is a positive one.

digestion system

plasmid            6ul
EcoRI              1ul
FD Buffer          2ul
H2O                11ul

Result: None of the clone picked is positive.

Analysis: Since no positive clones have been found, we come to check the principle and design of our experiment. We realize that if we use AP to treat with inserted fragments and vector, it is obvious that ligation can not talk place.