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- 14:07, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/12 October 2010 (New page: == Module I, Group 2c == Plasimds are extracted with TIANGEN plasimid extraction kit and digested with EcoR I to judge whether these clones are positive ones or not. ---- Digestion system...) (top)
- 14:02, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/10 October 2010 (New page: == Module I, Group 2c == Tranform the ligation product into DH5α.Then spread 100ul of transformation product on LB plate with Amp. Incubate the plate at 37 centidegree.) (top)
- 14:02, 27 October 2010 (diff | hist) Team:Tsinghua/Notebook/10 September 2010 (→Module I, Group 2c) (top)
- 14:02, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/11 October 2010 (New page: == Module I, Group 2c == Several clones are picked and cultured in 3ml LB with Amp. Incubate at 37 centidegree overnight.) (top)
- 14:02, 27 October 2010 (diff | hist) Team:Tsinghua/Notebook/11 September 2010 (→Module I, Group 2c) (top)
- 13:54, 27 October 2010 (diff | hist) Team:Tsinghua/Notebook/11 September 2010
- 13:50, 27 October 2010 (diff | hist) Team:Tsinghua/Notebook/10 September 2010
- 13:49, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/9 October 2010 (New page: == Module I, Group 2c == After purification, ligation reaction took place in a mixed system as following， respectively. eGFP 3ul PSB1C13 2ul T4 ligase(NE...)
- 13:39, 27 October 2010 (diff | hist) Team:Tsinghua/Notebook/8 October 2010 (→Module I, Group 2c)
- 13:39, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/8 October 2010 (New page: == Module I, Group 2c == Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I. ---- Double digestion system eGFP 5ul EcoR I 1ul Pst I ...)
- 13:34, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/29 September 2010 (New page: == Module I, Group 2c == Conduct PCR to amplify eGFP, mCherry, Kan and Chlr with DNA polymerase SuperMix. PCR system (SuperMix): H2O 8.5μl primer1 0.5μl primer2 0.5μl ...) (top)
- 13:32, 27 October 2010 (diff | hist) Team:Tsinghua/Notebook/17 August 2010 (→Module I, Group 2b) (top)
- 22:00, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/16 September 2010 (New page: == Module I, group 2c == PCR is run with ligation product as template. ---- PCR system ligation prodct 1.5ul Primer(up) 2ul Primer(Do) 2ul H2O ...) (top)
- 21:57, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/15 September 2010 (→Module I, group 2c) (top)
- 21:55, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/15 September 2010
- 21:49, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/15 September 2010 (New page: Since it seems as if no ligation product of three fragments appeared in the PCR identification result, we strongly doubt that there is something wrong with the DraIII cutting site, perhaps...)
- 21:25, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/14 September 2010 (New page: == Module I, group 2c == Repeat the PCR of yesterday.) (top)
- 21:24, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/13 September 2010 (New page: == Module I, group 2c == Since we have got no positive results for mutiple-fragment ligation, we really want to know the efficiency of it. Therefore, we use the ligation product as templa...) (top)
- 21:12, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/4 September 2010 (top)
- 21:06, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/3 September 2010 (New page: == Module I, group 2c == Ligation product is transformed in to DH5aα 24h after ligation.) (top)
- 21:05, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/2 September 2010 (→YX's part) (top)
- 21:01, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/1 September 2010 (top)
- 20:57, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/31 August 2010 (→Module I, group 2c) (top)
- 20:50, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/31 August 2010 (→Module I, group 2c)
- 20:50, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/31 August 2010
- 20:47, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/27 August 2010 (New page: == Module I, group 2c == As too many negative clones grown on the control plate, which means that vector has not been toally digested. To solve the problem, we decide to use gel purificati...) (top)
- 20:37, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/26 August 2010 (→Module I, Group 2c) (top)
- 20:34, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/24 August 2010 (→Module I, Group 2b) (top)
- 20:28, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/25 August 2010 (→Module I, group 2(b)) (top)
- 20:27, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/26 August 2010
- 20:25, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/24 August 2010 (→Module I, Group 2b)
- 20:20, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/23 August 2010 (→Module I, group 2c) (top)
- 20:19, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/23 August 2010 (→Module I, group 2c)
- 19:48, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/24 August 2010 (New page: == Module I, Group 2b == After purification, ligation reaction took place in a mixed system as following. eGFP 1ul mCherry 2ul Kan 1ul T4...)
- 19:43, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/16 August 2010 (→Module I, group 2c) (top)
- 19:43, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/23 August 2010 (→Module I, group 2c)
- 19:42, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/23 August 2010 (New page: == Module I, group 2c == Run the same PCR program as yesterday and purify the product with gel purification kit. ---- Digest eGFP with XbaI and EcoRI, Ap is uesd to phosphorylate the 5' e...)
- 19:36, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/16 August 2010 (→Module I, group 2c)
- 19:34, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/21 August 2010 (top)
- 19:30, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/21 August 2010 (New page: == Module I, group 2c == == Module I, group 2c == Extract plasmid from the clone we Picked yesterday. Digest the plasmid with EcoRI and run gel to identify whether the clone is a positiv...)
- 19:12, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/20 August 2010 (top)
- 19:10, 26 October 2010 (diff | hist) N Team:Tsinghua/Notebook/20 August 2010 (New page: == Module I, group 2c == Extract plasmid from the clone we Picked yesterday. Digest the plasmid with EcoRI and run gel to identify whether the clone is a positive one. digestion system ...)
- 19:06, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/19 August 2010 (top)
- 19:05, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/18 August 2010 (top)
- 19:04, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/17 August 2010
- 18:50, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/16 August 2010
- 18:37, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/7 August 2010 (→Module I, group 2c) (top)
- 18:35, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/7 August 2010 (→Module I, group 2(b))
- 17:10, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/2 August 2010 (top)
- 16:58, 26 October 2010 (diff | hist) Team:Tsinghua/Notebook/31 July 2010 (→Module I, Group 2c) (top)
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