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From 2010.igem.org

Module I, Group 1A

Colony pcr to screen the clones in which landing pad has successfully integrated into genome, using up primers from the plasmid and down primers from the genome. Select the positive clones to amplify in LB media with 0.03% tetracyclin.

Module I, Group 2c

After purification, ligation reaction took place in a mixed system as following， respectively.

eGFP                 3ul
PSB1C13              2ul
T4 ligase(NEB)       1ul
T4 ligation Buffer   2ul
H2O                  12ul

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mCherry              2ul
PSB1C13              2ul
T4 ligase(NEB)       1ul
T4 ligation Buffer   2ul
H2O                  13ul

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Kan                  3ul
PSB1C13              2ul
T4 ligase(NEB)       1ul
T4 ligation Buffer   2ul
H2O                  12ul

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Incubate at 16 centidegree overnight.