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From 2010.igem.org

Module I, Group 2c

Plasimds are extracted with TIANGEN plasimid extraction kit and digested with EcoR I to judge whether these clones are positive ones or not.

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Digestion system

plasmid        2ul
EcoR I         1ul
FD buffer      2ul
H2O            15ul

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Incubate at 37 centidegree for 2h, after which gel is run.

Result: one of clone for PE, two for PM and two for PK are positive ones.