Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/22

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E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/10/22 Friday (Naoto)

member
naoto and watachin

Experiment:Colony PCR

material
  • colony of E.coli
    • bcsA:1~22
    • bcsD:25~48
other materials were same as protocol3
procedure
see protocol3

Experiment:Electrophoresis

material
  • PCR production (Colony PCR)
you can see other materials at protocol4
procesure
refer to protocol4
result
From the length of bands
bcsA→No.2,9,11,
seem to be correct inserts

Preparation:making preculture for Miniprep

material
  • colony of E.coli
    • bcsA→No.2,9,11
  • LB+Cam broth
procedure
see protocol2


Experiment:Colony PCR

material
  • colony of E.coli
    • bcsB:1~24
    • bcsC:25~48
other materials were same as protocol3
procedure
see protocol3

Experiment:Electrophoresis

material
  • PCR production (Colony PCR)
you can see other materials at protocol4
procesure
refer to protocol4
result
we couldn't confirm inserts



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