Team:INSA-Lyon/Project/Stage3/Strategy/construction3

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Construction 3


First, two phasin proteins extracted from igem database and an intein protein (extract from?)are ligated to form the phasin-intein construction, kept in collection for a future ligation. A part of this phasin-intein construction is placed after a constitutive promoter in order to test the construction validity by fluorescence quantification.





Figure 1 : phasin-intein construction



Pbad/araC has to be placed upstream the cI gene and the phasin-intein construction,the productions of cI repressor and phasin and intein proteins are dependant of arabinose addition in medium.


Figure 2 : Construction under Pbad araC control






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