Team:INSA-Lyon/Project/Stage3/Strategy/construction3
From 2010.igem.org
Construction 3
First, two phasin proteins extracted from igem database and an intein protein (extract from?)are ligated to form the phasin-intein construction, kept in collection for a future ligation. A part of this phasin-intein construction is placed after a constitutive promoter in order to test the construction validity by fluorescence quantification.
![](https://static.igem.org/mediawiki/2010/5/5a/Construction3_2.png)
Figure 1 : phasin-intein construction
![](https://static.igem.org/mediawiki/2010/1/1c/Construction3_1.png)
Pbad/araC has to be placed upstream the cI gene and the phasin-intein construction,the productions of cI repressor and phasin and intein proteins are dependant of arabinose addition in medium.
![](https://static.igem.org/mediawiki/2010/4/46/Construction3_4.png)
Figure 2 : Construction under Pbad araC control
![](https://static.igem.org/mediawiki/2010/8/8d/Construction3_3.png)