Team:INSA-Lyon/Project/Stage3/Strategy/construction3
From 2010.igem.org
Construction for the regulation by arabinose
We can find below the details schemas of the three constructions requisite for this regulation
- LuxR and LuxI construction
- LuxR and HSL construction
- pBad and AraC construction
Construction3 : regulation under pBad and AraC protein
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First, two phasin proteins extracted from igem database and an intein protein are ligated to form the phasin-intein construction, kept in collection for a future ligation. A part of this phasin-intein construction is placed after a constitutive promoter in order to test the construction validity by fluorescence quantification.
Figure 1 : phasin-intein construction
Pbad/araC has to be placed upstream the cI gene and the phasin-intein construction,the productions of cI repressor and phasin and intein proteins are dependant of arabinose addition in medium.
Figure 2 : Construction under Pbad araC control
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