Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/30

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E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/8/30 Monday (Naoto)

Experiment:electrophoresis

member
NEX,bambi75 and watachin

material

  • PCR production (bcsC from E.coli)
  • 1×TAE buffer
  • agarose gel

procedure
see protocol4

result
bands of bcsC were appeared
201008301.jpg

Experiment:direct PCR

member
naoto

material

  • A.xylinum JCM7664strain

procedure
refer to protocol3

Experiment:electrophoresis

member
naoto

material

  • PCR production (bcsA from A.xylinum)
  • 1×TAE buffer
  • agarose gel

procedure
see protocol4


result
a band of bcsA were appeared (below bands appeared to be primer)
201008302.jpg

Experiment:purification of agarose gel

member
naoto

material

  • bcsA from A.xylinum in agarose gel
  • Gel Extraction kit(QIAGEN)

procedure
see protocol5