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Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/30
From 2010.igem.org
E.coli Fiber Project Notebook
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2010/8/30 Monday (Naoto)
Experiment:electrophoresis
member
NEX,bambi75 and watachin
material
- PCR production (bcsC from E.coli)
- 1×TAE buffer
- agarose gel
procedure
see protocol4
result
bands of bcsC were appeared
Experiment:direct PCR
member
naoto
material
- A.xylinum JCM7664strain
procedure
refer to protocol3
Experiment:electrophoresis
member
naoto
material
- PCR production (bcsA from A.xylinum)
- 1×TAE buffer
- agarose gel
procedure
see protocol4
result
a band of bcsA were appeared
(below bands appeared to be primer)
Experiment:purification of agarose gel
member
naoto
material
- bcsA from A.xylinum in agarose gel
- Gel Extraction kit(QIAGEN)
procedure
see protocol5
