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Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/22
From 2010.igem.org
(Difference between revisions)
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==2010/10/22 Friday (Naoto)== | ==2010/10/22 Friday (Naoto)== | ||
| - | '''member''' | + | :'''member''' |
| - | naoto and watachin | + | :naoto and watachin |
===Experiment:Colony PCR=== | ===Experiment:Colony PCR=== | ||
| - | '''material''' | + | :'''material''' |
| - | *colony of E.coli | + | :*colony of E.coli |
| - | **bcsA:1~22 | + | :**bcsA:1~22 |
| - | **bcsD:25~48 | + | :**bcsD:25~48 |
| - | other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | + | :other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> |
===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
| - | '''material''' | + | :'''material''' |
| - | *PCR production (Colony PCR) | + | :*PCR production (Colony PCR) |
| - | you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html> | + | :you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html> |
| - | '''procesure''' | + | :'''procesure''' |
| - | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html> | + | :refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html> |
| - | '''result''' | + | :'''result''' |
| - | From the length of bands | + | :From the length of bands |
| - | bcsA→No.2,9,11, | + | :bcsA→No.2,9,11, |
| - | seem to be correct inserts | + | :seem to be correct inserts |
===Preparation:making preculture for Miniprep === | ===Preparation:making preculture for Miniprep === | ||
| - | '''material''' | + | :'''material''' |
| - | *colony of ''E.coli'' | + | :*colony of ''E.coli'' |
| - | **bcsA→No.2,9,11 | + | :**bcsA→No.2,9,11 |
| - | *LB+Cam broth | + | :*LB+Cam broth |
| - | '''procedure''' | + | :'''procedure''' |
| - | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Grow up culture of E.coli">protocol2</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Grow up culture of E.coli">protocol2</a></html> |
===Experiment:Colony PCR=== | ===Experiment:Colony PCR=== | ||
| - | '''material''' | + | :'''material''' |
| - | *colony of E.coli | + | :*colony of E.coli |
| - | **bcsB:1~24 | + | :**bcsB:1~24 |
| - | **bcsC:25~48 | + | :**bcsC:25~48 |
| - | other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | + | :other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> |
===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
| - | '''material''' | + | :'''material''' |
| - | *PCR production (Colony PCR) | + | :*PCR production (Colony PCR) |
| - | you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html> | + | :you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html> |
| - | '''procesure''' | + | :'''procesure''' |
| - | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html> | + | :refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html> |
| - | '''result''' | + | :'''result''' |
| - | we couldn't confirm inserts | + | :we couldn't confirm inserts |
<br/> | <br/> | ||
Latest revision as of 18:06, 27 October 2010
E.coli Fiber Project Notebook
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2010/10/22 Friday (Naoto)
- member
- naoto and watachin
Experiment:Colony PCR
- material
- colony of E.coli
- bcsA:1~22
- bcsD:25~48
- colony of E.coli
- other materials were same as protocol3
- procedure
- see protocol3
Experiment:Electrophoresis
- material
- PCR production (Colony PCR)
- you can see other materials at protocol4
- procesure
- refer to protocol4
- result
- From the length of bands
- bcsA→No.2,9,11,
- seem to be correct inserts
Preparation:making preculture for Miniprep
- material
- colony of E.coli
- bcsA→No.2,9,11
- LB+Cam broth
- colony of E.coli
- procedure
- see protocol2
Experiment:Colony PCR
- material
- colony of E.coli
- bcsB:1~24
- bcsC:25~48
- colony of E.coli
- other materials were same as protocol3
- procedure
- see protocol3
Experiment:Electrophoresis
- material
- PCR production (Colony PCR)
- you can see other materials at protocol4
- procesure
- refer to protocol4
- result
- we couldn't confirm inserts
