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Team:INSA-Lyon/Project/Stage3/Strategy/construction3
From 2010.igem.org
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<img src="https://static.igem.org/mediawiki/2010/5/5a/Construction3_2.png"> | <img src="https://static.igem.org/mediawiki/2010/5/5a/Construction3_2.png"> | ||
</div> | </div> | ||
| + | <p style="text-align:center; font-size:0.9em;"><em> Figure 1 : phasin-intein construction</em></p> | ||
<p></p><br/> | <p></p><br/> | ||
<a href="https://static.igem.org/mediawiki/2010/1/1c/Construction3_1.png"> | <a href="https://static.igem.org/mediawiki/2010/1/1c/Construction3_1.png"> | ||
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| + | <div style="text-align:center;"> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/4/46/Construction3_4.png"> | ||
| + | </div> | ||
| + | <p style="text-align:center; font-size:0.9em;"><em> Figure 2 : Construction under Pbad araC control </em></p> | ||
| + | <p></p><br/> | ||
| + | <a href="https://static.igem.org/mediawiki/2010/1/1c/Construction3_1.png"> | ||
| + | <div style="text-align:left;"> | ||
| + | <img class="image1" src="https://static.igem.org/mediawiki/2010/8/8d/Construction3_3.png" | ||
| + | alt="" /></a> | ||
| + | </div> | ||
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<h3></h3><br> | <h3></h3><br> | ||
Revision as of 14:31, 27 October 2010
Construction 3
First, two phasin proteins extracted from igem database and an intein protein (extract from?)are ligated to form the phasin-intein construction, kept in collection for a future ligation. A part of this phasin-intein construction is placed after a constitutive promoter in order to test the construction validity by fluorescence quantification.
Figure 1 : phasin-intein construction
Pbad/araC has to be placed upstream the cI gene and the phasin-intein construction,the productions of cI repressor and phasin and intein proteins are dependant of arabinose addition in medium.
Figure 2 : Construction under Pbad araC control
