Team:INSA-Lyon/Project/Stage3/Strategy/construction3
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Revision as of 14:21, 27 October 2010
Construction 3
First, two phasin proteins extracted from igem database and an intein protein (extract from?)are ligated to form the phasin-intein construction, kept in collection for a future ligation. A part of this phasin-intein construction is placed after a constitutive promoter in order to test the construction validity by fluorescence quantification.
![](https://static.igem.org/mediawiki/2010/5/5a/Construction3_2.png)
![](https://static.igem.org/mediawiki/2010/1/1c/Construction3_1.png)
Pbad/araC has to be placed upstream the cI gene and the phasin-intein construction,the productions of cI repressor and phasin and intein proteins are dependant of arabinose addition in medium.