Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/20
From 2010.igem.org
(Difference between revisions)
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__NOTOC__ | __NOTOC__ | ||
==2010/9/20 Monday (watachin)== | ==2010/9/20 Monday (watachin)== | ||
- | ===Experiment: | + | ===Experiment:Electrophoresis=== |
- | '''Member'''<br /> | + | :'''Member'''<br /> |
- | naoto and watachin | + | :naoto and watachin |
- | '''Materials and Procedure''' | + | :'''Materials and Procedure''' |
- | + | :*PCR production(Colony PCR) | |
- | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis"> | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html> |
- | '''Result''' | + | :'''Result''' |
[[Image:2010-09-20-01.jpg]] | [[Image:2010-09-20-01.jpg]] | ||
Line 16: | Line 16: | ||
===Experiment:PCR=== | ===Experiment:PCR=== | ||
- | '''Member'''<br /> | + | :'''Member'''<br /> |
- | naoto and watachin | + | :naoto and watachin |
- | ''' | + | :'''Material and Procedure''' |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
+ | :bcs genes | ||
+ | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html> | ||
===Experiment:Plating=== | ===Experiment:Plating=== | ||
- | '''Member'''<br /> | + | :'''Member'''<br /> |
- | naoto and watachin | + | :naoto and watachin |
- | + | ||
- | + | ||
- | + | :'''Material and Procedure''' | |
+ | :plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth | ||
===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
- | '''Member'''<br /> | + | :'''Member'''<br /> |
- | naoto and watachin | + | :naoto and watachin |
- | + | :'''Result''' | |
- | '''Result''' | + | :Failure (I had the wrong protocol.) |
- | Failure (I had the wrong protocol.) | + | |
===Experiment:PCR=== | ===Experiment:PCR=== | ||
- | '''Member'''<br /> | + | :'''Member'''<br /> |
- | naoto | + | :naoto |
- | ''' | + | ::'''Material and Procedure''' |
- | PCR of bcs genes of A.xylinum and E.coli | + | :PCR of bcs genes of A.xylinum and E.coli |
- | + | ||
- | + | ||
+ | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html> | ||
===Experiment:Digestion=== | ===Experiment:Digestion=== | ||
- | '''Member'''<br /> | + | :'''Member'''<br /> |
- | watachin | + | :watachin |
- | ''' | + | :'''Material and Procedure''' |
- | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion"> | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">protocol7</a></html> |
===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
- | '''Member'''<br /> | + | :'''Member'''<br /> |
- | watachin | + | :watachin |
- | + | ||
- | + | ||
+ | :'''Material and Procedure''' | ||
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html> | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html> | ||
- | '''Result''' | + | :'''Result''' |
[[Image:2010-09-20-03.jpg]] | [[Image:2010-09-20-03.jpg]] | ||
- | ===Experiment:DNA | + | ===Experiment:DNA extraction from agarose gel=== |
- | '''Member'''<br /> | + | :'''Member'''<br /> |
- | watachin | + | :watachin |
- | ''' | + | :'''Material and Procedure''' |
- | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html> | |
- | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN"> | + | |
===Experiment:Ligation=== | ===Experiment:Ligation=== | ||
- | '''Member'''<br /> | + | :'''Member'''<br /> |
- | watachin | + | :watachin |
- | ''' | + | :'''Material and Procedure''' |
- | + | ||
- | + | ||
- | + | ||
+ | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">tprotocol8</a></html> | ||
===Experiment:Transformation=== | ===Experiment:Transformation=== | ||
- | '''Member'''<br /> | + | :'''Member'''<br /> |
- | watachin | + | :watachin |
- | + | ||
- | + | ||
+ | :'''Material and Procedure''' | ||
- | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation"> | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">protocol9</a></html> |
Revision as of 13:27, 27 October 2010
![](https://static.igem.org/mediawiki/2010/9/9f/Re-main.png)
E.coli Fiber Project Notebook
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2010/9/20 Monday (watachin)
Experiment:Electrophoresis
- Member
- naoto and watachin
- Materials and Procedure
- PCR production(Colony PCR)
- follow protocol4
- Result
Experiment:PCR
- Member
- naoto and watachin
- Material and Procedure
- bcs genes
- follow protocol3
Experiment:Plating
- Member
- naoto and watachin
- Material and Procedure
- plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
Experiment:Electrophoresis
- Member
- naoto and watachin
- Result
- Failure (I had the wrong protocol.)
Experiment:PCR
- Member
- naoto
- Material and Procedure
- PCR of bcs genes of A.xylinum and E.coli
- follow protocol3
Experiment:Digestion
- Member
- watachin
- Material and Procedure
- follow protocol7
Experiment:Electrophoresis
- Member
- watachin
- Material and Procedure
follow the protocol(Electrophoresis)
- Result
Experiment:DNA extraction from agarose gel
- Member
- watachin
- Material and Procedure
- follow protocol5
Experiment:Ligation
- Member
- watachin
- Material and Procedure
- follow tprotocol8
Experiment:Transformation
- Member
- watachin
- Material and Procedure
- follow protocol9