Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/20

From 2010.igem.org


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/9/20 Monday (watachin)

Experiment:Electrophoresis

Member
naoto and watachin
Materials and Procedure
  • Colony PCR production(bcsC,bcsD)
follow protocol4
Result

2010-09-20-01.jpg 2010-09-20-02.jpg

Experiment:PCR

Member
naoto and watachin
Material and Procedure
  • templete DNA:bcsA,B and D
follow protocol3

Experiment:Plating

Member
naoto and watachin
Material and Procedure
  • bcs genes in E.coli(Ecos JM109)
  • LB + cam medium

Experiment:Electrophoresis

Member
naoto and watachin
Result
Failure (I had the wrong protocol.)

Experiment:PCR

Member
naoto
Material and Procedure
  • templete DNA:A.xylinum in OpenWetWare broth
follow protocol3

Experiment:Digestion

Member
watachin
Material and Procedure
follow protocol7

Experiment:Electrophoresis

Member
watachin
Material and Procedure
follow protocol
Result

2010-09-20-03.jpg

Experiment:DNA extraction from agarose gel

Member
watachin
Material and Procedure
follow protocol5

Experiment:Ligation

Member
watachin
Material and Procedure
follow tprotocol8

Experiment:Transformation

Member
watachin
Material and Procedure
follow protocol9
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