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Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/16
From 2010.igem.org
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==2010/08/16 Monday(NEX)== | ==2010/08/16 Monday(NEX)== | ||
| - | ===Experiment:Agarose | + | ===Experiment:Agarose gel Electrophoresis=== |
| - | '''Member'''<br /> | + | :'''Member'''<br /> |
| - | NEX and watachin | + | :NEX and watachin |
| - | '''Materials'''<br /> | + | :'''Materials'''<br /> |
| - | *bcsA,B,C(digestion had been done last Friday) | + | :*bcsA,B,C(digestion had been done last Friday) |
| - | *Agarose Gel | + | :*1% Agarose Gel |
| - | *10×Loading buffer | + | :*10×Loading buffer |
| - | '''Procedure''' | + | :'''Procedure''' |
| - | #Pour the Agarose gel onto gel plate and make wells on it. | + | :#Pour the Agarose gel onto gel plate and make wells on it. |
| - | #Apply the 10×Loading buffer in the one of wells. | + | :#Apply the 10×Loading buffer in the one of wells. |
| - | #Apply the Insert DNA in another well. | + | :#Apply the Insert DNA in another well. |
| - | '''Consequence'''<br /> | + | :'''Consequence'''<br /> |
| - | We could see | + | :We could see a band of bcsB.<br/> |
[[Image:20100816.jpg]] | [[Image:20100816.jpg]] | ||
Latest revision as of 21:33, 26 October 2010
E.coli Fiber Project Notebook
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2010/08/16 Monday(NEX)
Experiment:Agarose gel Electrophoresis
- Member
- NEX and watachin
- Materials
- bcsA,B,C(digestion had been done last Friday)
- 1% Agarose Gel
- 10×Loading buffer
- Procedure
- Pour the Agarose gel onto gel plate and make wells on it.
- Apply the 10×Loading buffer in the one of wells.
- Apply the Insert DNA in another well.
- Consequence
- We could see a band of bcsB.
