Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/20
From 2010.igem.org
Line 1: | Line 1: | ||
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}} | {{:Team:Tokyo_Metropolitan/Notebook/Fiber}} | ||
- | + | __NOTOC__ | |
==2010/9/20 Monday (watachin)== | ==2010/9/20 Monday (watachin)== | ||
===Experiment:Insert check Electrophoresis=== | ===Experiment:Insert check Electrophoresis=== |
Revision as of 17:42, 24 October 2010
![](https://static.igem.org/mediawiki/2010/9/9f/Re-main.png)
E.coli Fiber Project Notebook
|
|
|
2010/9/20 Monday (watachin)
Experiment:Insert check Electrophoresis
Member
naoto and watachin
Materials and Procedure
follow the protocol(Electrophoresis)
Result
Experiment:PCR
Member
naoto and watachin
Materials and Procedure
Direct PCR of bcs genes
follow the protocol(Direct PCR)
Experiment:Plating
Member
naoto and watachin
Materials and Procedure
plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
Experiment:Electrophoresis
Member
naoto and watachin
Result
Failure (I had the wrong protocol.)
Experiment:PCR
Member
naoto
Materials and Procedure
PCR of bcs genes of A.xylinum and E.coli
follow the protocol(Direct_PCR)
Experiment:Digestion
Member
watachin
Materials and Procedure
follow the protocol(Digestion)
Experiment:Electrophoresis
Member
watachin
Materials and Procedure
follow the protocol(Electrophoresis)
Result
Experiment:DNA purification from agarose gel with QIAGEN
Member
watachin
Materials and Procedure
follow the protocol(DNA purification from agarose gel with QIAGEN)
Experiment:Ligation
Member
watachin
Materials and Procedure
follow the protocol(Ligation)
Experiment:Transformation
Member
watachin
Materials and Procedure