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Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/30
From 2010.igem.org
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bands of bcsC were appeared<br/> | bands of bcsC were appeared<br/> | ||
[[Image:201008301.jpg]] | [[Image:201008301.jpg]] | ||
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===Experiment:direct PCR=== | ===Experiment:direct PCR=== | ||
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'''material'''<br/> | '''material'''<br/> | ||
| - | *''A.xylinum'' | + | *''A.xylinum'' JCM7664strain |
'''procedure'''<br/> | '''procedure'''<br/> | ||
| - | + | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | |
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===Experiment:electrophoresis=== | ===Experiment:electrophoresis=== | ||
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'''procedure'''<br/> | '''procedure'''<br/> | ||
| - | + | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | |
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'''result'''<br/> | '''result'''<br/> | ||
a band of bcsA were appeared | a band of bcsA were appeared | ||
| - | (below bands | + | (below bands appeared to be primer)<br/> |
[[Image:201008302.jpg]] | [[Image:201008302.jpg]] | ||
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'''material'''<br/> | '''material'''<br/> | ||
*bcsA from ''A.xylinum'' in agarose gel | *bcsA from ''A.xylinum'' in agarose gel | ||
| - | * | + | *QIAGEN Gel Extraction kit |
'''procedure'''<br/> | '''procedure'''<br/> | ||
| - | + | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html> | |
Revision as of 20:47, 23 October 2010
E.coli Fiber Project Notebook
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Contents |
2010/8/30 Monday (Naoto)
Experiment:electrophoresis
member
NEX,bambi75 and watachin
material
- PCR production (bcsC from E.coli)
- 1×TAE buffer
- agarose gel
procedure
follow protocol4
result
bands of bcsC were appeared
Experiment:direct PCR
member
naoto
material
- A.xylinum JCM7664strain
procedure
refer to protocol3
Experiment:electrophoresis
member
naoto
material
- PCR production (bcsA from A.xylinum)
- 1×TAE buffer
- agarose gel
procedure
see protocol4
result
a band of bcsA were appeared
(below bands appeared to be primer)
Experiment:purification of agarose gel
member
naoto
material
- bcsA from A.xylinum in agarose gel
- QIAGEN Gel Extraction kit
procedure
