Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/16

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(Experiment:Make Agarose Gels Electro-phoresis)
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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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==2010/08/16 Monday(NEX)==
==2010/08/16 Monday(NEX)==
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===Experiment:Make Agarose Gels Electro-phoresis===
 
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'''Member'''<br />
 
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NEX and watachin
 
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'''Materials'''<br />
 
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*1×TaE 200ml
 
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*Agarose 2g
 
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*Ethidium bromide 10µl
 
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'''Procedure'''
 
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#Add and mix above materials in an Erlenmeyer flask.
 
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#Warm them in a Microwave oven.
 
===Experiment:Agarose Gel Electro-phoresis===
===Experiment:Agarose Gel Electro-phoresis===
'''Member'''<br />
'''Member'''<br />
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Same avobe
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NEX and watachin
'''Materials'''<br />
'''Materials'''<br />
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*Insert DNA
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*PCR production
*Agarose Gel
*Agarose Gel
*10×Loading buffer 5μl
*10×Loading buffer 5μl

Revision as of 18:21, 22 October 2010


E.coli Fiber Project Notebook

August 2010
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2010/08/16 Monday(NEX)

Experiment:Agarose Gel Electro-phoresis

Member
NEX and watachin

Materials

  • PCR production
  • Agarose Gel
  • 10×Loading buffer 5μl

Procedure

  1. Pour the Agarose gel onto gel plate and make wells on it.
  2. Apply the 10×Loading buffer in the one of wells.
  3. Apply the Insert DNA in another well.

Consequence
We could see the bcsB.

20100816.jpg