Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/20
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Revision as of 13:29, 27 October 2010
E.coli Fiber Project Notebook
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2010/9/20 Monday (watachin)
Experiment:Electrophoresis
- Member
- naoto and watachin
- Materials and Procedure
- Colony PCR production(bcsC,bcsD)
- follow protocol4
- Result
Experiment:PCR
- Member
- naoto and watachin
- Material and Procedure
- bcsA,B,D
- follow protocol3
Experiment:Plating
- Member
- naoto and watachin
- Material and Procedure
- plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
Experiment:Electrophoresis
- Member
- naoto and watachin
- Result
- Failure (I had the wrong protocol.)
Experiment:PCR
- Member
- naoto
- Material and Procedure
- PCR of bcs genes of A.xylinum and E.coli
- follow protocol3
Experiment:Digestion
- Member
- watachin
- Material and Procedure
- follow protocol7
Experiment:Electrophoresis
- Member
- watachin
- Material and Procedure
follow the protocol(Electrophoresis)
- Result
Experiment:DNA extraction from agarose gel
- Member
- watachin
- Material and Procedure
- follow protocol5
Experiment:Ligation
- Member
- watachin
- Material and Procedure
- follow tprotocol8
Experiment:Transformation
- Member
- watachin
- Material and Procedure
- follow protocol9