Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/16

(Difference between revisions)

Latest revision as of 21:33, 26 October 2010

E.coli Fiber Project Notebook

Member

NEX and watachin

Materials

Procedure

Consequence

We could see a band of bcsB.

@@ Line 1: / Line 1: @@
+{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
 ==2010/08/16 Monday(NEX)==
-===Experiment:Make Agarose Gels Electro-phoresis===
-'''Member'''<br />
-NEX and watachin
-'''Materials'''<br />
+===Experiment:Agarose gel Electrophoresis===
-*1×TaE 200ml
+:'''Member'''<br />
-*Agarose 2g
+:NEX and watachin
-*Ethidium bromide　10µl
-'''Procedure'''
+:'''Materials'''<br />
-#Add and mixture above materials in an Erlenmeyer flask
+:*bcsA,B,C(digestion had been done last Friday)
-#Warm up them in a Microwave oven
+:*1% Agarose Gel
+:*10×Loading buffer
-===Experiment:Agarose Gel Electro-phoresis===
+:'''Procedure'''
-'''Member'''<br />
+:#Pour the Agarose gel onto gel plate and make wells on it.
-Same avobe
+:#Apply the 10×Loading buffer in the one of wells.
+:#Apply the Insert DNA in another well.
-'''Materials'''<br />
+:'''Consequence'''<br />
-*Insert　DNA
+:We could see a band of bcsB.<br/>
-*Agarose Gel
-*10×Loading buffer 5μl
-'''Procedure'''
+[[Image:20100816.jpg]]
-#Pour the Agarose gel in a box and make dents on it
-#Pour the 10×Loading buffer in the one of dents
-#Pour the Insert DNA in another dent.
-'''Consequence'''<br />
-We could see the bcsB.<br/>
-[[Image:20100816.jpg]]
+<br/>