Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/30

From 2010.igem.org

(Difference between revisions)

Latest revision as of 17:39, 24 October 2010

E.coli Fiber Project Notebook

2010/8/30 Monday (Naoto)

Experiment:electrophoresis

member
NEX,bambi75 and watachin

material

PCR production (bcsC from E.coli)
1×TAE buffer
agarose gel

procedure
see　protocol4

result
bands of bcsC were appeared

Experiment:direct PCR

member
naoto

material

A.xylinum JCM7664strain

procedure
refer　to protocol3

Experiment:electrophoresis

member
naoto

material

PCR production (bcsA from A.xylinum)
1×TAE buffer
agarose gel

procedure
see　protocol4

result
a band of bcsA were appeared (below bands appeared to be primer)

Experiment:purification of agarose gel

member
naoto

material

bcsA from A.xylinum in agarose gel
Gel Extraction kit(QIAGEN)

procedure
see　protocol5

@@ Line 1: / Line 1: @@
+{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
+__NOTOC__
 ==2010/8/30 Monday (Naoto)==
 ===Experiment:electrophoresis===
@@ Line 10: / Line 12: @@
 '''procedure'''<br/>
-follow to protocol4
+see　<html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
 '''result'''<br/>
-bands of bcsC was appeared
+bands of bcsC were appeared<br/>
+[[Image:201008301.jpg]]
-===Experiment:separation of ''A.xylinus''===
-'''member'''<br/>
-naoto
-'''material'''<br/>
-*''A.xylinus'' in open wet ware media(8/25 made)
-'''procedure'''<br/>
-#take 0.5ml of ''A.xylinus'' in open wet ware media to tubes
-#centrifuge 15000rpm/5min
 ===Experiment:direct PCR===
@@ Line 31: / Line 23: @@
 '''material'''<br/>
-*A.xylinus separated above experiment
+*''A.xylinum'' JCM7664strain
 '''procedure'''<br/>
-follow to protocol3
+refer　to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
-===Experiment:purification of agarose gel===
-'''member'''<br/>
-NEX,bambi75 and watachin
-'''material'''<br/>
-*bcsC from E.coli in agarose gel
-*QIAGEN
-'''procedure'''<br/>
-follow to protocol5
 ===Experiment:electrophoresis===
@@ Line 52: / Line 33: @@
 '''material'''<br/>
-*PCR production (bcsA from A.xylinus)
+*PCR production (bcsA from A.xylinum)
 *1×TAE buffer
 *agarose gel
 '''procedure'''<br/>
-follow to protocol4
+see　<html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
 '''result'''<br/>
-a band of bcsA was appeared
+a band of bcsA were appeared
-(below bands appreared to be primer)
+(below bands appeared to be primer)<br/>
+[[Image:201008302.jpg]]
 ===Experiment:purification of agarose gel===
@@ Line 68: / Line 51: @@
 '''material'''<br/>
-*bcsA from A.xylinus in agarose gel
+*bcsA from ''A.xylinum'' in agarose gel
-*QIAGEN
+*Gel Extraction kit(QIAGEN)
 '''procedure'''<br/>
-follow to protocol5
+see　<html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>
+<br/>