Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/30

From 2010.igem.org

(Difference between revisions)
(New page: ==2010/8/30 Monday (Naoto)== ===Experiment:electrophoresis=== '''member'''<br/> NEX,bambi75 and watachin '''material'''<br/> *PCR production (bcsC from E.coli) *1×TAE buffer *agarose gel...)
 
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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
==2010/8/30 Monday (Naoto)==
==2010/8/30 Monday (Naoto)==
===Experiment:electrophoresis===
===Experiment:electrophoresis===
Line 10: Line 12:
'''procedure'''<br/>
'''procedure'''<br/>
-
follow to protocol4
+
see　<html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
'''result'''<br/>
'''result'''<br/>
-
bands of bcsC was appeared
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bands of bcsC were appeared<br/>
-
 
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[[Image:201008301.jpg]]
-
===Experiment:separation of ''A.xylinus''===
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-
'''member'''<br/>
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-
naoto
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-
 
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-
'''material'''<br/>
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*''A.xylinus'' in open wet ware media(8/25 made)
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-
 
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-
'''procedure'''<br/>
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#take 0.5ml of ''A.xylinus'' in open wet ware media to tubes
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#centrifuge 15000rpm/5min
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===Experiment:direct PCR===
===Experiment:direct PCR===
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'''material'''<br/>
'''material'''<br/>
-
*A.xylinus separated above experiment
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*''A.xylinum'' JCM7664strain
'''procedure'''<br/>
'''procedure'''<br/>
-
follow to protocol3
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refer　to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
-
 
+
-
===Experiment:purification of agarose gel===
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-
'''member'''<br/>
+
-
NEX,bambi75,watachin
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-
 
+
-
'''material'''<br/>
+
-
*bcsC from E.coli in agarose gel
+
-
*QIAGEN
+
-
 
+
-
'''procedure'''<br/>
+
-
follow to protocol5
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===Experiment:electrophoresis===
===Experiment:electrophoresis===
Line 52: Line 33:
'''material'''<br/>
'''material'''<br/>
-
*PCR production (bcsA from A.xylinus)
+
*PCR production (bcsA from A.xylinum)
*1×TAE buffer
*1×TAE buffer
*agarose gel
*agarose gel
'''procedure'''<br/>
'''procedure'''<br/>
-
follow to protocol4
+
see　<html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
 +
 
'''result'''<br/>
'''result'''<br/>
-
a band of bcsA was appeared
+
a band of bcsA were appeared
-
(below bands appreared to be primer)
+
(below bands appeared to be primer)<br/>
 +
[[Image:201008302.jpg]]
===Experiment:purification of agarose gel===
===Experiment:purification of agarose gel===
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'''material'''<br/>
'''material'''<br/>
-
*bcsA from A.xylinus in agarose gel
+
*bcsA from ''A.xylinum'' in agarose gel
-
*QIAGEN
+
*Gel Extraction kit(QIAGEN)
'''procedure'''<br/>
'''procedure'''<br/>
-
follow to protocol5
+
see　<html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<br/>

Latest revision as of 17:39, 24 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
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October 2010
SUNMONTUEWEDTHUFRISAT
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3 4 5 6 7 8 9
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24 25 26 27 28 29 30
31

2010/8/30 Monday (Naoto)

Experiment:electrophoresis

member
NEX,bambi75 and watachin

material

  • PCR production (bcsC from E.coli)
  • 1×TAE buffer
  • agarose gel

procedure
see　protocol4

result
bands of bcsC were appeared
201008301.jpg

Experiment:direct PCR

member
naoto

material

  • A.xylinum JCM7664strain

procedure
refer　to protocol3

Experiment:electrophoresis

member
naoto

material

  • PCR production (bcsA from A.xylinum)
  • 1×TAE buffer
  • agarose gel

procedure
see　protocol4


result
a band of bcsA were appeared (below bands appeared to be primer)
201008302.jpg

Experiment:purification of agarose gel

member
naoto

material

  • bcsA from A.xylinum in agarose gel
  • Gel Extraction kit(QIAGEN)

procedure
see　protocol5




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