Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/20
From 2010.igem.org
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:'''Material and Procedure''' | :'''Material and Procedure''' | ||
- | :*bcsA,B | + | :*templete DNA:bcsA,B and D |
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html> | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html> | ||
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:'''Material and Procedure''' | :'''Material and Procedure''' | ||
- | : | + | :*bcs genes in E.coli(Ecos JM109) |
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===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
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:'''Material and Procedure''' | :'''Material and Procedure''' | ||
- | : | + | :*templete DNA:A.xylinum in OpenWetWare broth |
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:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html> | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html> | ||
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- | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis"> | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol</a></html> |
:'''Result''' | :'''Result''' |
Latest revision as of 13:38, 27 October 2010
![](https://static.igem.org/mediawiki/2010/9/9f/Re-main.png)
E.coli Fiber Project Notebook
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2010/9/20 Monday (watachin)
Experiment:Electrophoresis
- Member
- naoto and watachin
- Materials and Procedure
- Colony PCR production(bcsC,bcsD)
- follow protocol4
- Result
Experiment:PCR
- Member
- naoto and watachin
- Material and Procedure
- templete DNA:bcsA,B and D
- follow protocol3
Experiment:Plating
- Member
- naoto and watachin
- Material and Procedure
- bcs genes in E.coli(Ecos JM109)
- LB + cam medium
Experiment:Electrophoresis
- Member
- naoto and watachin
- Result
- Failure (I had the wrong protocol.)
Experiment:PCR
- Member
- naoto
- Material and Procedure
- templete DNA:A.xylinum in OpenWetWare broth
- follow protocol3
Experiment:Digestion
- Member
- watachin
- Material and Procedure
- follow protocol7
Experiment:Electrophoresis
- Member
- watachin
- Material and Procedure
- follow protocol
- Result
Experiment:DNA extraction from agarose gel
- Member
- watachin
- Material and Procedure
- follow protocol5
Experiment:Ligation
- Member
- watachin
- Material and Procedure
- follow tprotocol8
Experiment:Transformation
- Member
- watachin
- Material and Procedure
- follow protocol9