Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/22

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
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==2010/10/22 Friday (Naoto)==
 +
:'''member'''
 +
:naoto and watachin
 +
 +
===Experiment:Colony PCR===
 +
:'''material'''
 +
 +
:*colony of E.coli
 +
:**bcsA:1~22
 +
:**bcsD:25~48
 +
:other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
 +
 +
:'''procedure'''
 +
 +
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
 +
 +
===Experiment:Electrophoresis===
 +
:'''material'''
 +
 +
:*PCR production (Colony PCR)
 +
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
 +
 +
:'''procesure'''
 +
 +
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
 +
 +
:'''result'''
 +
 +
:From the length of bands
 +
 +
:bcsA→No.2,9,11,
 +
 +
:seem to be correct inserts
 +
 +
===Preparation:making preculture for Miniprep ===
 +
:'''material'''
 +
 +
:*colony of ''E.coli''
 +
:**bcsA→No.2,9,11
 +
:*LB+Cam broth
 +
 +
:'''procedure'''
 +
 +
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Grow up culture of E.coli">protocol2</a></html>
 +
 +
 +
===Experiment:Colony PCR===
 +
:'''material'''
 +
 +
:*colony of E.coli
 +
:**bcsB:1~24
 +
:**bcsC:25~48
 +
:other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
 +
 +
:'''procedure'''
 +
 +
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
 +
 +
===Experiment:Electrophoresis===
 +
:'''material'''
 +
 +
:*PCR production (Colony PCR)
 +
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
 +
 +
:'''procesure'''
 +
 +
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
 +
 +
:'''result'''
 +
 +
:we couldn't confirm inserts
 +
 +
 +
 +
<br/>

Latest revision as of 18:06, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
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3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31


2010/10/22 Friday (Naoto)

member
naoto and watachin

Experiment:Colony PCR

material
  • colony of E.coli
    • bcsA:1~22
    • bcsD:25~48
other materials were same as protocol3
procedure
see protocol3

Experiment:Electrophoresis

material
  • PCR production (Colony PCR)
you can see other materials at protocol4
procesure
refer to protocol4
result
From the length of bands
bcsA→No.2,9,11,
seem to be correct inserts

Preparation:making preculture for Miniprep

material
  • colony of E.coli
    • bcsA→No.2,9,11
  • LB+Cam broth
procedure
see protocol2


Experiment:Colony PCR

material
  • colony of E.coli
    • bcsB:1~24
    • bcsC:25~48
other materials were same as protocol3
procedure
see protocol3

Experiment:Electrophoresis

material
  • PCR production (Colony PCR)
you can see other materials at protocol4
procesure
refer to protocol4
result
we couldn't confirm inserts



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