Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/22
From 2010.igem.org
(Difference between revisions)
(→Experiment:Electrophoresis) |
|||
Line 35: | Line 35: | ||
seem to be correct inserts | seem to be correct inserts | ||
+ | |||
+ | ===Preparation:making preculture for Miniprep === | ||
+ | '''material''' | ||
+ | |||
+ | *colony of ''E.coli'' | ||
+ | **bcsA→No.2,9,11 | ||
+ | *LB+Cam broth | ||
+ | |||
+ | '''procedure''' | ||
+ | |||
+ | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Grow up culture of E.coli">protocol2</a></html> | ||
+ | |||
===Experiment:Colony PCR=== | ===Experiment:Colony PCR=== |
Revision as of 17:25, 25 October 2010
![](https://static.igem.org/mediawiki/2010/9/9f/Re-main.png)
E.coli Fiber Project Notebook
|
|
|
2010/10/22 Friday (Naoto)
member naoto and watachin
Experiment:Colony PCR
material
- colony of E.coli
- bcsA:1~22
- bcsD:25~48
other materials were same as protocol3
procedure
see protocol3
Experiment:Electrophoresis
material
- PCR production (Colony PCR)
you can see other materials at protocol4
procesure
refer to protocol4
result
From the length of bands
bcsA→No.2,9,11,
seem to be correct inserts
Preparation:making preculture for Miniprep
material
- colony of E.coli
- bcsA→No.2,9,11
- LB+Cam broth
procedure
see protocol2
Experiment:Colony PCR
material
- colony of E.coli
- bcsB:1~24
- bcsC:25~48
other materials were same as protocol3
procedure
see protocol3
Experiment:Electrophoresis
material
- PCR production (Colony PCR)
you can see other materials at protocol4
procesure
refer to protocol4
result
we couldn't confirm inserts