Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/08

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==2010/9/8 Monday (NEX)==
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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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===Experiment:digestion of bcsA,B,C(from''E.coli'')===
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==2010/09/08 Monday (NEX)==
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'''member'''<br />
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===Experiment:Digestion of bcsA,B,C===
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bambi73 and NEX
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:'''Member'''<br />
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:bambi75 and NEX
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'''material'''<br />
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:'''Material'''<br />
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*bcsA,B,C(from ''E.coli'')
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:*bcsA,B,C
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*10×Mbuffer
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:*10×Mbuffer
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*BSA
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:*BSA
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*XbaI
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:*XbaI
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*SpeI
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:*SpeI
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'''procedure'''<br />
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:'''Procedure'''<br />
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#add "material" to PCR tubes(show below)
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:#Add "material" to PCR tubes(show below)
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#incubation at (37℃,7h)
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:#Incubation at (37℃,7h)
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{|class="wikitable" style="text-align:right"
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:{|class="wikitable" style="text-align:right"
|+composition
|+composition
! !!bcsA!!bcsB!!bcsC!!
! !!bcsA!!bcsB!!bcsC!!
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|}
|}
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===Experiment:Make Agarose Gels Electro-phoresis===
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===Preparation:Make Agarose Gels Electro-phoresis===
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'''Member'''<br />
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:'''Member'''<br />
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Same above
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:Same above
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'''Materials'''<br />
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:'''Materials'''<br />
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*1×TaE 200ml
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:*1×TaE 200ml
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*Agarose 2g
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:*Agarose 2g
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*Ethidium bromide　10µl
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:*Ethidium bromide　10µl
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'''Procedure'''
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:'''Procedure'''
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#Add and mixture above materials in an Erlenmeyer flask
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:#Add and mixture above materials in an Erlenmeyer flask
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#Warm up them in a Microwave oven
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:#Warm up them in a Microwave oven
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<br/>

Latest revision as of 06:38, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/09/08 Monday (NEX)

Experiment:Digestion of bcsA,B,C

Member
bambi75 and NEX
Material
  • bcsA,B,C
  • 10×Mbuffer
  • BSA
  • XbaI
  • SpeI
Procedure
  1. Add "material" to PCR tubes(show below)
  2. Incubation at (37℃,7h)
composition
bcsAbcsBbcsC
solution of bcs(μl) 606060
10×Mbuffer(μl) 6 6 6
BSA(μl) 2 2
XbaI(μl) 1.21.2
SpeI(ul) 1.21.2

Preparation:Make Agarose Gels Electro-phoresis

Member
Same above
Materials
  • 1×TaE 200ml
  • Agarose 2g
  • Ethidium bromide　10µl
Procedure
  1. Add and mixture above materials in an Erlenmeyer flask
  2. Warm up them in a Microwave oven

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