From 2010.igem.org
E.coli Fiber Project Notebook
2010/09/08 Monday (NEX)
Experiment:Digestion of bcsA,B,C
- Member
- bambi75 and NEX
- Material
- bcsA,B,C
- 10×Mbuffer
- BSA
- XbaI
- SpeI
- Procedure
- Add "material" to PCR tubes(show below)
- Incubation at (37℃,7h)
composition
| | bcsA | bcsB | bcsC |
|
| solution of bcs(μl)
| 60 | 60 | 60 |
|
| 10×Mbuffer(μl)
| 6 | 6 | 6 |
|
| BSA(μl)
| | 2 | 2 |
|
| XbaI(μl)
| | 1.2 | 1.2 |
|
| SpeI(ul)
| 1.2 | 1.2 | |
|
Preparation:Make Agarose Gels Electro-phoresis
- Member
- Same above
- Materials
- 1×TaE 200ml
- Agarose 2g
- Ethidium bromide 10µl
- Procedure
- Add and mixture above materials in an Erlenmeyer flask
- Warm up them in a Microwave oven
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