Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/08
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(New page: ==2010/9/8 Monday (NEX)== ===Experiment:digestion of bcsA,B,C,D(''A.xylinus'')=== '''member'''<br /> bambi73 and NEX '''material'''<br /> *bcsA,B,C(from ''E.coli'') *10×Mbuffer *BSA ...) |
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- | + | {{:Team:Tokyo_Metropolitan/Notebook/Fiber}} | |
- | ===Experiment: | + | ==2010/09/08 Monday (NEX)== |
- | ''' | + | ===Experiment:Digestion of bcsA,B,C=== |
- | + | :'''Member'''<br /> | |
+ | :bambi75 and NEX | ||
- | ''' | + | :'''Material'''<br /> |
- | *bcsA,B,C | + | :*bcsA,B,C |
- | *10×Mbuffer | + | :*10×Mbuffer |
- | *BSA | + | :*BSA |
- | *XbaI | + | :*XbaI |
- | *SpeI | + | :*SpeI |
- | ''' | + | :'''Procedure'''<br /> |
- | # | + | :#Add "material" to PCR tubes(show below) |
- | # | + | :#Incubation at (37℃,7h) |
- | {|class="wikitable" style="text-align:right" | + | :{|class="wikitable" style="text-align:right" |
|+composition | |+composition | ||
! !!bcsA!!bcsB!!bcsC!! | ! !!bcsA!!bcsB!!bcsC!! | ||
Line 29: | Line 30: | ||
|- | |- | ||
! XbaI(μl) | ! XbaI(μl) | ||
- | | || | + | | ||1.2||1.2|| |
|- | |- | ||
! SpeI(ul) | ! SpeI(ul) | ||
- | | | + | | 1.2||1.2|| || |
|} | |} | ||
+ | ===Preparation:Make Agarose Gels Electro-phoresis=== | ||
+ | :'''Member'''<br /> | ||
+ | :Same above | ||
- | + | :'''Materials'''<br /> | |
- | ''' | + | :*1×TaE 200ml |
- | + | :*Agarose 2g | |
+ | :*Ethidium bromide 10µl | ||
- | ''' | + | :'''Procedure''' |
- | + | :#Add and mixture above materials in an Erlenmeyer flask | |
- | + | :#Warm up them in a Microwave oven | |
- | + | ||
- | + | <br/> | |
- | + | ||
- | + |
Latest revision as of 06:38, 27 October 2010
E.coli Fiber Project Notebook
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2010/09/08 Monday (NEX)
Experiment:Digestion of bcsA,B,C
- Member
- bambi75 and NEX
- Material
- bcsA,B,C
- 10×Mbuffer
- BSA
- XbaI
- SpeI
- Procedure
- Add "material" to PCR tubes(show below)
- Incubation at (37℃,7h)
composition bcsA bcsB bcsC solution of bcs(μl) 60 60 60 10×Mbuffer(μl) 6 6 6 BSA(μl) 2 2 XbaI(μl) 1.2 1.2 SpeI(ul) 1.2 1.2
Preparation:Make Agarose Gels Electro-phoresis
- Member
- Same above
- Materials
- 1×TaE 200ml
- Agarose 2g
- Ethidium bromide 10µl
- Procedure
- Add and mixture above materials in an Erlenmeyer flask
- Warm up them in a Microwave oven