Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/08
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Revision as of 16:59, 24 October 2010
E.coli Fiber Project Notebook
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2010/9/8 Monday (NEX)
Experiment:digestion of bcsA,B,C
member
bambi75 and NEX
material
- bcsA,B,C
- 10×Mbuffer
- BSA
- XbaI
- SpeI
procedure
- add "material" to PCR tubes(show below)
- incubation at (37℃,7h)
bcsA | bcsB | bcsC | ||
---|---|---|---|---|
solution of bcs(μl) | 60 | 60 | 60 | |
10×Mbuffer(μl) | 6 | 6 | 6 | |
BSA(μl) | 2 | 2 | ||
XbaI(μl) | 1.2 | 1.2 | ||
SpeI(ul) | 1.2 | 1.2 |
Preparation:Make Agarose Gels Electro-phoresis
Member
Same above
Materials
- 1×TaE 200ml
- Agarose 2g
- Ethidium bromide 10µl
Procedure
- Add and mixture above materials in an Erlenmeyer flask
- Warm up them in a Microwave oven