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Team:Caltech/Week 9 - 2010.igem.org

Team:Caltech/Week 9

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iGEM 2010

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Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15

Monday 8/9

Group meeting!
Re-ran two gels of CPCR results
- Successful: 55-5, 56-1/-2/-3, 57-1/-2/-3, 54-5/-6
- Prepped DNA for use in digestions tomorrow

Tuesday 8/10

Digested DNA for ligations:
- L31: L17 + <partinfo>K215000</partinfo> + Kan
- L32: L19 + L20 + Tet
- L33: L19 + L18 + Tet
- L34: L17 + L21 + Amp
- L35: L18 + L21 + Amp
- L41: L26 + <partinfo>B0015</partinfo> + Tet
- L49: HSP + L40 + Tet
- L60: <partinfo>K215000</partinfo> + L57 + Tet
- L61: <partinfo>K215000</partinfo> + L56 + Tet
- L63: L29 + L20 + Amp
- L64: L27 + L20 + Amp
Prepped 37-1, 54-5/-6, 55-5, 56, 57 DNA
Started CPCR of L36, L38
- All colonies failed
Started liquid culture of <partinfo>I15008</partinfo>

Wednesday 8/11

<partinfo>I15008</partinfo> culture did not grow; will re-pick more tomorrow.
L43 plate had no colonies; will redo L43 tomorrow.
Began CPCR of all successful ligations.

Thursday 8/12

Friday 8/13

Weekend 8/14-8/15

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