Our project aims to use dual-wavelength light transduction to produce arbitrary 3D shapes of polyhydroxybutyrate (PHB) plastic from soybean oil. Using a radical crosslinking reagent, we will crosslink PHB oligomers in cell lysate, released upon a light-induced lysis gene network. We hope to apply this capability to three dimensional printing, offering a cheap alternative to current rapid-prototyping technologies. We also plan to apply special consideration to the ramifications of possible commercial enterprises developed in iGEM competitions with open source materials, such as BioBricks.
Specific goals include: