Team:Caltech/Week 15

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Monday 9/20

  • Transformation of completed sigma32 (HtpR) RFC10 BioBrick construction into electrocompetent DH5 alpha cells

Tuesday 9/21

  • Miniprep of DNA from Monday's transformed cells

Wednesday 9/22

  • Verify that miniprepped plasmid DNA is of sufficiently high concentration to send out for sequencing
  • Send out plasmid DNA for sequencing

Thursday 9/23

Friday 9/24

  • Sequencing apparently showed that L36 and L37 did not ligate properly at the second stage of PCR ligation.


 
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