Team:Caltech/Week 6

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Monday 7/19

  • Group meeting!
    • Discussed issues with cloning and next week's focus.
  • Performed a digest (now for 2hr) and ligations (L18-23). Transformed into DH5α.
  • Turns out that the sequencing primers we ordered (G00100 & G00101) are incorrect - they appear to bind on the wrong sides of the insert. We ordered new primers designed to bind to the BioBrick prefix and suffix.
  • Ran gel of digest product to verify correct bands.
Digest test gel with US and DS digested bricks. (7/19)

Tuesday 7/20

  • All ligation transformations failed. Will try 16hr ligations and purifying digest products with a PCR purification kit.
  • Made new growth curves testing AIBN effect on cells in LB
  • Performed another live/dead assay
    • Had solutions of cells in glycerol and AIBN
    • Plated cells every hour for three hours
    • Combined glycerol solutions with LB and plated the solution


Wednesday 7/21

  • Ran another digest:

Thursday 7/22

  • Ran gel of digest products from yesterday to verify correctness of the digestion.
Digest test gel with upstream, downstream, and backbone digested bricks. (7/22)

Friday 7/23

Weekend 7/24-25

 
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