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Team:Caltech/Week 6 - 2010.igem.org
 

Team:Caltech/Week 6

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iGEM 2010



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Monday 7/19

  • Group meeting!
    • Discussed issues with cloning and next week's focus.
  • Performed a digest (now for 2hr) and ligations (L18-23). Transformed into DH5α.
  • Ran gel of digest product to verify correct bands.
Digest test gel with US and DS digested bricks. (7/19)

Tuesday 7/20

  • All ligation transformations failed. Will try 16hr ligations and purifying digest products with a PCR purification kit.
  • Made new growth curves testing AIBN effect on cells in LB
  • Performed another live/dead assay
    • Had solutions of cells in glycerol and AIBN
    • Plated cells every hour for three hours
    • Combined glycerol solutions with LB and plated the solution


Wednesday 7/21

Thursday 7/22

Friday 7/23

Weekend 7/24-25

 
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