Team:Caltech/Week 12

From 2010.igem.org

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*Use a different plate of L62-1 cells (HSP-LacI-GFP), pick 3 different colonies and re-perform the HSP-LacI-GFP
*Use a different plate of L62-1 cells (HSP-LacI-GFP), pick 3 different colonies and re-perform the HSP-LacI-GFP
**Only use 0 mM Glucose and 5 mM Glucose
**Only use 0 mM Glucose and 5 mM Glucose
 +
*Make new digests of L54 (HSP-LacI-RBS), E0430 (EYFP), and RFP-TetR backbone
 +
*Ligate L54 to L56 (Lysis-Terminator), L54 to L57 (Another Lysis-Terminator), and L54 to E0430
 +
*Transform aforementioned ligations into cells, along with L90 and L91 (both HSP-Lysis)
 +
*Attempt PCR extraction and amplification of E. coli sigma 32 (HtpR)
==Tuesday 8/31==
==Tuesday 8/31==

Revision as of 20:59, 31 August 2010


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Monday 8/30

  • Use a different plate of L62-1 cells (HSP-LacI-GFP), pick 3 different colonies and re-perform the HSP-LacI-GFP
    • Only use 0 mM Glucose and 5 mM Glucose
  • Make new digests of L54 (HSP-LacI-RBS), E0430 (EYFP), and RFP-TetR backbone
  • Ligate L54 to L56 (Lysis-Terminator), L54 to L57 (Another Lysis-Terminator), and L54 to E0430
  • Transform aforementioned ligations into cells, along with L90 and L91 (both HSP-Lysis)
  • Attempt PCR extraction and amplification of E. coli sigma 32 (HtpR)

Tuesday 8/31

  • Fluorescence spectrometry shows that cells in 5 mM glucose produces significantly less GFP than those in no glucose growth medium.

Wednesday 9/1

Thursday 9/2

Friday 9/3

 
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