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Team:Caltech/Week 11 - 2010.igem.org
 

Team:Caltech/Week 11

From 2010.igem.org

(Difference between revisions)
(New page: {{Caltech_iGEM_10| Content= {{:Team:Caltech/WeekTemplate}} ==Monday 8/23== ==Tuesday 8/24== ==Wednesday 8/25== ==Thursday 8/26== ==Friday 8/27==   }})
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==Monday 8/23==
==Monday 8/23==
 +
*Ligate L90, L91, and L92 (overnight ligation)
==Tuesday 8/24==
==Tuesday 8/24==
 +
*Transform the ligation product from yesterday
==Wednesday 8/25==
==Wednesday 8/25==
 +
*Pick colonies from the transformed cells. Perform colony PCR and run gel on the PCR product.
 +
**Gel shows negative result Failed
 +
*Set up HSP-LacI-GFP experiment with glucose concentration (.1mM, .5mM, 1mM, 5mM)
==Thursday 8/26==
==Thursday 8/26==
 +
*Glucose appears to not effect the GFP expression in this experiment which contradicts the result from previous experiment.
==Friday 8/27==
==Friday 8/27==

Revision as of 20:09, 31 August 2010


iGEM 2010



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Monday 8/23

  • Ligate L90, L91, and L92 (overnight ligation)

Tuesday 8/24

  • Transform the ligation product from yesterday

Wednesday 8/25

  • Pick colonies from the transformed cells. Perform colony PCR and run gel on the PCR product.
    • Gel shows negative result Failed
  • Set up HSP-LacI-GFP experiment with glucose concentration (.1mM, .5mM, 1mM, 5mM)

Thursday 8/26

  • Glucose appears to not effect the GFP expression in this experiment which contradicts the result from previous experiment.

Friday 8/27

 
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